mCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of the Discosoma species. It is a monomeric form that has been designed with multiple cycles of mutation, directed modification, and evolutionary selection.
It exhibits an excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry shows high photostability and resistance to photobleaching. It also shows improved gloss and an extremely fast maturation rate. Anti-mCherry, clone1C51 is an affinity-purified antibody that recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref .: Schaner, NC et al. (2004). Nat. Biotechnol. 22 (12): 1567-72).
Clone 1C51 detects the mCherry protein in multiple species.
His-tagged full-length recombinant MCherry.
1. Anti-mCherry antibody, clone IC51, cat. No. MAB131873 detects the mCherry protein and is tested for use in immunocytochemistry and Western blotting.
2. Western Blot Analysis: A representative lot detected mCherry in Western Blot applications (Krajcovic, M., et. Al. (2013). Mol Biol Cell. 24 (23): 3736-45).
3. Western blot analysis: a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry versus non-transfected HEK293 cells (courtesy of Dr. Gerry Shaw).
4. Immunocytochemical analysis: a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry (courtesy of Dr. Gerry Shaw).
~ 32 kDa observed; 26.72 kDa calculated. Uncharacterized bands can be seen in some lysates.
Mouse monoclonal antibody purified IgG2a in buffer containing PBS with 0.03% sodium azide with 50% glycerol.
Evaluated by Western Blot in HEK293 cells transfected with pFin-EF1-mCherry. Western blot analysis: 1 µg / ml of this antibody detected mCherry in 10 µg of lysate from HEK293 cells transfected with pFin-EF1-mCherry.