Product name: Rapid Cytosol / Particle Separation Kit
Example type: Tissue, adherent cells, cells in suspension
Test type: Direct
Test time: 1h 00m
Product description
Abcam’s Rapid Particle / Cytosol Separation Kit physically separates the cytosol from the particle compartments rapidly through an oil layer and therefore the two fractions do not contact or diffuse with each other. With the method, contaminations can be avoided even for small molecules. Subcellular localization and analysis of the interesting factors can be performed with precision.
Notes
The location and translocation of proteins, signaling molecules, and other small molecules within cells regulate cell growth, differentiation, and many other cellular functions. The separation of cytosol and particle fractions is an important step in the study of the subcellular localization of cellular components. However, traditional methods often take hours to separate the cytosol from the particles, so that some cellular components, especially small molecules, and metabolites, diffuse or redistribute during the separation procedure.
Relevance
Cytosol/particle separation is the method of separating the cytosol and particle fractions. The location and translocation of proteins, signaling molecules, and other small molecules within cells regulate cell growth, differentiation, and many other cellular functions. The separation of cytosol and particle fractions is an important step in the study of the subcellular localization of cellular components.
Features and Benefits
• Simple procedure; takes less than 1 hour
• Quick and convenient
• The new Rapid Cytosol / Particle Separation Kit physically separates the cytosol from the particle comparators rapidly through an oil layer and therefore the two fractions do not contact or diffuse with each other. With the method, contaminations can be avoided even for small molecules.
Kit components
• Cell suspension buffer
• Cytosol releasing buffer
• Oil layer
• Particle layer
Storage conditions: -20 ° C
Shipping conditions: Gel Pack
