Comparison of the sensitivity of Western blotting between PVDF and NC membranes

Comparison of the sensitivity of Western blotting between PVDF and NC membranes
Western blotting (WB) is likely one of the most generally used methods to establish proteins in addition to publish translational modifications of proteins. The number of electroblotted membrane is likely one of the key elements affecting the detection sensitivity of the protein which is transferred from gel to membrane in WB. The commonest used membranes are polyvinylidene fluoride (PVDF) and nitrocellulose (NC) membranes.
Which membrane of those two is extra appropriate for WB has not been reported thus far. Right here, by incubating proteins which have been transferred to PVDF or NC membranes with a collection of antibodies and various kinds of lectins, we investigated the connection between the binding potential of those two membranes to proteins or glycoproteins and the molecular weight of the goal protein. The antibody re-probed potential of the 2 membranes was additionally explored.
Furthermore, we verified the above outcomes by instantly incubating proteins having completely different molecular weights onto PVDF or NC membranes. Certain proteins have been stained with direct blue-71, and the staining depth was quantitated by scanning and densitometry.

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